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13C-1H correlated HSQC NMR spectra of the repeating unit trisaccharide of the lfnA mutant (A) and of the polysaccharide of the complemented lfnA mutant (B). Circles highlight the position in panel B of the characteristic methyl signal of the acetamidino group, which is not observed in the lfnA mutant. (C) Deduced structure of the lfnA mutant O polysaccharide. (D) Structure of the wild-type (or complemented lfnA mutant) O polysaccharide.
