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Stability of CAT as a function of the growth phase. Exponential-phase cells (open symbols) and stationary-phase cells [ATCC 35319(pPSVthl)] (filled symbols) were incubated at 32°C in a culture medium supplemented with tetracycline (20 mg liter−1). CAT activity (squares) and total cell protein concentrations (triangles) were determined at specific time intervals. The symbols indicate the means of three experiments, and the error bars indicate the standard deviations.
