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SDS-PAGE analysis of the purification of recombinant LuxAB. Lanes: 1, crude extract; 2, ammonium sulfate fraction (35 to 60%); 3, purified by DEAE-Sepharose chromatography; 4, extract purified by Sephacryl-200 chromatography. P. leiognathi luciferase is a heterodimeric protein with the α subunit (∼40.5 kDa) and β subunit (∼38 kDa).
