TABLE 1.
Purification of recombinant LuxG and His6-tagged LuxG from E. coli TUNER(DE3)/pLacI
| Purification step | Total protein (mg) | Total activitya (U) | Sp act (U mg−1) | Yield (%) |
|---|---|---|---|---|
| Purification of recombinant His6-tagged LuxG | ||||
| Crude extract | 1,500 | 2,457 | 1.64 | 100 |
| Ni2+ column | 109 | 2,680 | 24.7 | 109 |
| Purification of recombinant LuxG | ||||
| Crude extract | 759 | 2,705 | 3.6 | 100 |
| DEAE-Sepharose | 190 | 2,732 | 14.3 | 101 |
| 20 to 40% (NH4)2SO4 precipitation | 83 | 1,567 | 18.9 | 58 |
| Sephacryl S200 | 38.4 | 803 | 20.9 | 30 |
a
One unit (U) is the amount of enzyme that catalyzes the oxidation of 1 μmol of NADH min−1.