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Purification of recombinant LuxAB from E. coli BL21(DE3)
| Purification step | Total protein (mg) | Total activitya (1018 photons) | Sp act (1014 photons mg−1) |
|---|---|---|---|
| Crude extract | 2,093 | 1.3 | 6.2 |
| 20 to 40% (NH4)2SO4 precipitation | 1,795 | 6.3 | 35.1 |
| DEAE-Sepharose | 1,080 | 4.2 | 38.9 |
| Sephacryl S200 | 480 | 2.5 | 52.1 |
The total photons from each assay were calculated by integrating the area under the luminescence trace and converting the value into quantum units by correlating them with the luminol reaction (34).
