Growth of the coculture in the fermentor. In the preculture, around 30% of the I. hospitalis cells were occupied by N. equitans cells (two to four per Ignicoccus cell). (A) Growth curves. I. hospitalis cells, •; total N. equitans cells, shaded ×; attached N. equitans cells, plain shaded square; free N. equitans cells, unshaded ×. (B) Numbers of N. equitans cells (ranging from 0 to 10) attached to cells of I. hospitalis are represented by no shading (n = 0) increasing to the darkest shading (n = 10). The insert is an enlarged view of the cell concentrations within the first 5 h after inoculation.