TABLE 1.
Plasmids used in this study
| Plasmid | Relevant feature(s)a | Reference or source |
|---|---|---|
| pTZ18cm− | High-copy-no. cloning vector, cm | Trenzyme |
| pCR2.1 | High-copy-no. cloning vector, amp | Invitrogen |
| pNCO113 | High-copy-no. expression vector, amp | 40 |
| pTrcHisA | High-copy-no. expression vector, amp | Invitrogen |
| pXI12 | pBR322 sacB5′ ermAM cryT RBS PvegI sacB3′ amp, B. subtilis integration/expression vector | 18 |
| pBEST501 | B. subtilis integration vector, neo amp | 21 |
| pBluescriptII SK− | High-copy-no. cloning vector, amp | Stratagene |
| pSG01 | pTrcHisA containing a 0.960-kbp BamHI/PstI fragment carrying S. davawensis ribCopt | This study |
| pSG02 | pNCO113 containing a 0.960-kbp NcoI/HindIII fragment carrying S. davawensis ribC | This study |
| pSG03 | pTrcHisA containing a 0.948-kbp BamHI/PstI fragment carrying B. subtilis ribC | This study |
| pSG05 | pCR2.1 containing an internal 0.5-kbp S. coelicolor ribC (SCO5711) fragment | This study |
| pSG06 | pTZ18cm− containing a 5,837-bp S. davawensis genomic fragment (ribC locus) | This study |
| pSG10 | pIXI12 containing a 0.960-kbp NdeI/XmaI fragment carrying S. davawensis ribCopt | This study |
| pSG60 | pBluescript SK− containing a 2.609-kbp XhoI/SpeI fragment carrying the B. subtilis ribC locus; ribC of this locus was replaced by neo from pBEST501 | This study |
a
Abbreviations: cm, chloramphenicol resistance cassette; amp, ampicillin resistance cassette; sacB, integration of genes by homologous double-crossover recombination at B. subtilis sacB; ermAM, erythromycin resistance cassette; neo, neomycin resistance cassette; PvegI, medium-strength vegI promoter; ribC, flavokinase/FAD synthetase gene.