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Reactivation experiments with DAAO (EC 1.4.3.3)
| Cofactora | DAAO activityb |
|---|---|
| FAD (Sigma-Aldrich) | 4.62 |
| FAD (S. davawensis RibC) | 4.55 |
| RoFAD (S. davawensis RibC) | 0 |
| FAD, without enzyme | 0 |
| None, without enzyme | 0 |
| Nondialyzed enzyme | 45.5 |
FAD and RoFAD were used at 40 μM.
Activity is expressed as nanomoles of benzoylformic acid produced per minute per milligram of protein.
