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UV spectra of (A) 75 μM protocatechuate and 33 μM protocatechuyl-CoA in 50 mM potassium phosphate buffer (pH 6.8) and (B) 4 μM protocatechuyl-CoA at different pH values. The maximum absorption of protocatechuyl-CoA at an alkaline pH in the near-UV region is at 345 nm. The enzyme activity was measured photometrically at pH 8.5 and 365 nm using a filter photometer with a strong 365-nm light source (Hg spectral line).
