TABLE 1.

Various aromatic acid-CoA ligase activities in extracts of T. aromatica after anaerobic growth with nitrate on different aromatic substrates

Enzyme	Activities (nmol min−1 mg−1) after growth on the following substratesa:
	Benzoate	4-Hydroxybenzoate	3-Hydroxybenzoate	Catechol
Benzoate-CoA ligase	166	101	85	91
4-Hydroxybenzoate-CoA ligase	<5	284	168	120
3-Hydroxybenzoate-CoA ligase	<5	<5	175	150
Protocatechuate-CoA ligase	<5	110	214	282

^aThe values are means of two individual cultures, and the deviations are less than ±15%. For the specific activities of the purified 3-hydroxybenzoate- and 4-hydroxybenzoate-CoA ligases, see the text. The benzoate-CoA ligase activities were as follows (33): with benzoate, 16.5 μmol min⁻¹ mg⁻¹; with 3-hydroxybenzoate, <1 μmol min⁻¹ mg⁻¹; with 4-hydroxybenzoate, <1 μmol min⁻¹ mg⁻¹; and with protocatechuate, <1 μmol min⁻¹ mg⁻¹.