TABLE 1.
Enzyme | Activities (nmol min−1 mg−1) after growth on the following substratesa:
|
|||
---|---|---|---|---|
Benzoate | 4-Hydroxybenzoate | 3-Hydroxybenzoate | Catechol | |
Benzoate-CoA ligase | 166 | 101 | 85 | 91 |
4-Hydroxybenzoate-CoA ligase | <5 | 284 | 168 | 120 |
3-Hydroxybenzoate-CoA ligase | <5 | <5 | 175 | 150 |
Protocatechuate-CoA ligase | <5 | 110 | 214 | 282 |
The values are means of two individual cultures, and the deviations are less than ±15%. For the specific activities of the purified 3-hydroxybenzoate- and 4-hydroxybenzoate-CoA ligases, see the text. The benzoate-CoA ligase activities were as follows (33): with benzoate, 16.5 μmol min−1 mg−1; with 3-hydroxybenzoate, <1 μmol min−1 mg−1; with 4-hydroxybenzoate, <1 μmol min−1 mg−1; and with protocatechuate, <1 μmol min−1 mg−1.