TABLE 2.
Plasmids used in this study
| Plasmid | Genotype and characteristics | Source/reference |
|---|---|---|
| pPZP300 | Strr Spcr; carries extra copies of the gene encoding the transcription factor VirG for increased virulence gene induction | 28 |
| pTrcB2 | Strr Spcr; pTrc200 derivative for lacIq/trc promoter-controlled expression of virB2 | 42 |
| pTrcB5 | Strr Spcr; pTrc200 derivative for lacIq/trc promoter-controlled expression of virB5 | 43 |
| pK*mobsacB | KanrsacB; mobilizable vector for the construction of in-frame deletion variants | 41 |
| pT7-H6TrxFus | Carbr; T7 promoter-controlled expression of hexahistidyl-thioredoxin (TrxA) fusion proteins | 29 |
| pT7-H6TrxVirB5 | Carbr; for expression of hexahistidyl-thioredoxin-VirB5 fusion protein | This work |
| pT7-StrepII | Carbr; T7 promoter-controlled expression of StrepII fusion proteins | 53 |
| pT7-7StrepIIVirB5 | Carbr; for expression of StrepII-VirB5 fusion protein | This work |
| pT7-7tzs | Carbr; T7 promoter-controlled expression of Tzs | 27 |
| pAS2 | Carbr; for expression of fusions with the GAL4 DNA binding domain; selection in yeast in the absence of Trp | 15 |
| pACTII | Carbr; for expression of fusions with the GAL4 activation domain; selection in yeast in the absence of Leu | 15 |
| pAS2-VirB5 | Carbr; virB5 in pAS2 | This work |
| pAS2-VirE2 | Carbr; virE2 in pAS2 | 8 |
| pAS2-Tzs | Carbr; tzs in pAS2 | This work |
| pACTII-VirB5 | Carbr; virB5 in pACTII | This work |
| pACTII-VirE2 | Carbr; virE2 in pACTII | 8 |
| pACTII-Tzs | Carbr; tzs in pACTII | This work |