TABLE 3.
Oligonucleotides used in this study
| Application | Name | Sequence and restriction sitea |
|---|---|---|
| Construction of pT7-7 StrepIIVirB5 | VirB5-5′ | 5′-CAGGGTACCCAGTTCGTTGTCAGCGATCCGGCG-3′ |
| and pT7-7H6TrxA-VirB5 | VirB5-3′ | 5′-GAGCTGCAGTCAGGGGACGGCCCCAAAGATG-3′ |
| Construction of pAS2-Tzs and | AS2-Tzs-5′ | 5′-GGAGGCTCATGATACTCCATCTCATCTACGGACC-3′ |
| pACTII-Tzs | AS2-Tzs-3′ | 5′-GACGGGGATCCTCACCGAATTCGCGTCAGCGTG-3′ |
| Cloning of virB2 | B2-5 | 5′-CCACACGAATTCCAAGTCGTGATGGACCGTCTCGA-3′ |
| B2-3 | 5′-CCACACGAATTCGACGGCAACGTGCATTGCGCATTT-3′ | |
| Deletion of virB2 | ΔB2-5 | 5′-AGGAGGTCCGCAATAATGAATGATCGTCTGGAAGCAACCCTT-3′ |
| ΔB2-3 | 5′-TTATTGCGGACCTCCTTGATTTAAGTCGAACAAGAGTTGATCGTC-3′ | |
| Cloning of virB8 | B8-5 | 5′-CGCAGTCTAGAGCAAAGTGGATCGGGCAACTTAT-3′ |
| B8-3 | 5′-CGCAGTCTAGACCTCTGCTCTCTGTTGATATTGCGCTT-3′ | |
| Deletion of virB8 | ΔB8-5 | 5′-CCGTGCTCGAGTTATTCAGACCCCTTCATGGCGACCACCT-3′ |
| ΔB8-3 | 5′-CCGTGCTCGAGATGACGAAAAAAGCATTTCTCA-3′ |
a
Restriction enzyme cleavage sites are underlined.