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Analysis of VirB protein and Tzs interactions using the yeast two-hybrid system
| Combination of vectors | Strength of interaction assessed by the no. of blue coloniesa |
|---|---|
| pAS2-VirB5/pACTII-VirB5 | ++ |
| pAS2-VirE2/pACTII-VirE2 | +++ |
| pAS2-Tzs/pACTII-Tzs | − |
| pAS2-VirB5/pACTII-Tzs | ++ |
| pAS2-Tzs/pACTII-VirB5 | − |
| pAS2-VirB5/pACTII-E2 | +++ |
| pAS2-Tzs/pACTII-VirE2 | − |
Relative numbers of β-galactosidase-producing colonies; six transformants each were tested from six independent transformation experiments.
