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. 2007 Dec 12;82(4):1908–1922. doi: 10.1128/JVI.01716-07

FIG. 1.

FIG. 1.

Influence of Myc tag and cell permeabilization on subcellular localization and immunocytochemical accessibility of HHV-8-encoded proteins. (A) Myc-tagged and untagged HHV-8-encoded proteins were expressed in HeLa cells and detected with either specific antibodies against the different HHV-8 proteins (nontagged proteins) or against the Myc tag. As a negative control, cells were transfected with the vector control (pcDNA4-Myc/His) and stained with antibodies against the viral proteins. Secondary antibodies were conjugated with Alexa Fluor 488. Cells permeabilized by Triton X-100 (B) and saponin (C) were subjected to immunocytochemical analysis of Myc-tagged K8, ORF20, and ORF54. For staining an antibody against the Myc tag was used. Secondary antibodies were conjugated with Alexa Fluor 488. No differences in localization and staining sensitivity were observed under the conditions used. Pictures were obtained using an epifluorescence microscope. The bar in K2 represents 10 μm. The same magnification was used in all panels.