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NF-κB activation with Myc-tagged and untagged K13. HeLa cells were cotransfected with reporter plasmid (pNF-κB-Luc; 1 μg) and increasing concentrations of effector plasmids encoding Myc-tagged or untagged K13. Activation of the NF-κB promoter was analyzed by luciferase measurement 48 h after transfection. Values were adjusted to total protein content, and the results are expressed in terms of the relative increase in induction in comparison with the negative control (0 μg).
