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. 2007 Dec 5;82(4):1739–1747. doi: 10.1128/JVI.01723-07

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FIG. 3. — More infectious EBV is produced in LMP2B-overexpressing Akata cells than in the control. Isolated CBMC were infected with supernatant from Akata-vector control, Akata-LMP2A, or Akata-LMP2B pools 1 to 3 collected 24 h after stimulation by BCR cross-linking. The transformation capacity was monitored by counting the wells after 6 weeks, at which time growth and clustering of cells indicated transformation. The percentage of transformation was calculated by setting 10 wells showing signs of transformation to 100% and by normalization to the transformation capacity of the supernatant of the corresponding BCR non-cross-linked control cells, representing the spontaneous EBV lytic activation in Akata cells. Means and SD are from three independent stimulation experiments with subsequent collection of supernatant and infection of CBMC. t tests were performed with a 95% confidence interval. *, no wells showed signs of transformation.