TABLE 1.
Characteristics of Akata cells useda
| Cell line | Vector construct | % sIgG+ cells | % Cells activated by gp350/220 staining
|
||
|---|---|---|---|---|---|
| 0 h | 24 h
|
||||
| Cross-linked | Not cross-linked | ||||
| Akatab | None | 96 ± 1 | ND | ND | ND |
| Akata-vectorb | pEneo | 92 ± 2 | 0.30 ± 0.11 | 4.62 ± 0.06 | 0.58 ± 0.09 |
| Akata-LMP2Bc | pEneo-FLAG-LMP2B | 88 ± 4 | 0.36 ± 0.20 | 5.42 ± 0.94 | 0.69 ± 0.23 |
| Akata-LMP2Ab | pEneo-LMP2A | 92 ± 1 | 0.24 ± 0.05 | 1.00 ± 0.81 | 0.34 ± 0.13 |
a
ND, not detected.
b
Values are presented as means ± SD from three independent experiments.
c
Values are presented as means ± SD from pools 1 to 3.