TABLE 2.
Copy numbers of viral RNA per CCID50 of EV71 mutants
EV71 strain | No. of copies of viral genome (in 100 μl)a | CCID50 (in 100 μl) | No. of copies of viral genome/CCID50 |
---|---|---|---|
BrCr-TR | 3.3 × 109 | 3.1 × 106 | 1.1 × 103 |
Nagoya | 3.4 × 109 | 1.8 × 107 | 1.9 × 102 |
Nagoya-2876A | 2.0 × 1010 | 5.6 × 106 | 3.6 × 103 |
Nagoya-2876A-S1 | 1.7 × 1010 | 4.8 × 106 | 3.5 × 103 |
Nagoya-2876A-3′ | 1.8 × 1010 | 2.4 × 106 | 7.5 × 103 |
Nagoya-2876A-S1-3′ | 1.4 × 1010 | 2.4 × 106 | 5.8 × 103 |
Viral RNA was extracted from each virus solution (prepared as crude cell lysates of infected RD cells), and then the number of copies of viral RNA was determined using real-time PCR.