TABLE 1.
Neutralization assay of H5N1 virus with monoclonal antibodies
| Group | H5N1 virusa | Clade | Neutralization titer of the indicated MAb by classb
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I
|
II
|
III
|
|||||||||||||||||
| 20A11 | 16F13 | 13D4 | 16G3 | 14E4 | 20H2 | 3C8 | 9H12B | 16A12 | 14D4 | 1G2 | 3F1B8 | 16F8 | 4GE1 | 1D8 | 6CF3 | 10DD2 | |||
| A | Ck/HKYU22/02 | 8 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 |
| Ck/IDN/2A/04 | 2.1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 800 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 1600 | ≥3200 | ≥3200 | ≥3200 | |
| Dk/IDN/MS/04 | 2.1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | 800 | ≥3200 | ≥3200 | ≥3200 | |
| Ck/Salatiga/BBVet1/05 | 2.1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | |
| Ck/Malang/BBVet4/04 | 2.1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | |
| HK213/03 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 1600 | < | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | < | |
| Ck/Bantul/BBVet1/05 | 2.1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | |
| Ck/YN/115/04 | 2.4 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 1600 | < | ≥3200 | ≥3200 | ≥3200 | 800 | ≥3200 | ≥3200 | ≥3200 | |
| B | Gs/GX/2112/04 | 5 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | 400 | < | ≥3200 | < | < | < | < |
| Ck/Jogjakarta/BBVet-IX/04 | 2.1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | |
| Dk/VNM/283/05 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | < | < | ≥3200 | < | 400 | < | |
| Rb pochard/HK/821/02 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | 1600 | ≥3200 | < | < | ≥3200 | < | < | < | |
| Ck/MYS/5858/04 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 1600 | ≥3200 | 800 | < | < | < | < | < | < | < | |
| VNM/1194/04 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 1600 | 1600 | 1600 | < | < | < | < | < | < | < | < | |
| VNM/1203/04 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | < | < | < | < | |
| Dk/VNM/N-XX/04 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | < | < | < | < | |
| Dk/VNM/S654/05 | 1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | < | < | < | |
| MDk/JX/1653/05 | 9 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | 400 | < | ≥3200 | < | < | < | < | |
| Dk/FJ/897/05 | 9 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | < | < | ≥3200 | < | < | < | < | |
| Gs/GY/337/06 | 4 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | < | < | < | |
| Gs/YN/5539/05 | 7 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | < | < | < | |
| C | IDN/5/05 | 2.1 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | 800 | < | 800 | 1600 |
| BH goose/QH15/05 | 2.2 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | 800 | ≥3200 | ≥3200 | ≥3200 | |
| Gf/ST/1341/06 | 2.2 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | ≥3200 | ≥3200 | |
| MDk/JX/2295/05 | 2.2 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | 800 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | |
| Ck/HN/999/05 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | ≥3200 | ≥3200 | ≥3200 | < | ≥3200 | < | ≥3200 | |
| Dk/HN/157/05 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | ≥3200 | ≥3200 | ≥3200 | 800 | ≥3200 | ≥3200 | ≥3200 | |
| Ck/ST/4231/03 | 2.2 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 400 | < | ≥3200 | ≥3200 | ≥3200 | 400 | ≥3200 | ≥3200 | ≥3200 | |
| Dk/GX/951/05 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | 800 | < | 400 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 800 | |
| Dk/HN/1265/05 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | 400 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 800 | ≥3200 | ≥3200 | |
| D | Ck/GX/2439/04 | 5 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | ≥3200 | < | ≥3200 | < | < | < | < |
| Qa/Gx/575/05 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | 400 | < | < | < | ≥3200 | < | < | < | < | ≥3200 | < | |
| Common Magpie/HK/645/2006 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ND | < | < | < | 800 | < | ≥3200 | < | < | < | < | |
| Oriental Magpie Robin/HK/366/2006 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | 800 | < | ≥3200 | < | < | < | < | |
| Ck/HK/282/2006 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | 400 | < | ≥3200 | < | < | < | < | |
| White eye/HK/737/07 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | ≥3200 | < | ≥3200 | < | < | < | < | |
| SZ/406H/2006 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | 1600 | 1600 | < | ≥3200 | < | < | < | < | |
| Dk/VNM/568/05 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | < | < | < | < | < | < | < | < | < | < | |
| Peregrine-falcon/HK/1143/07 | 2.3 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | ≥3200 | < | 800 | < | < | < | < | < | ≥3200 | < | < | < | |
| Dk/HN/101/04 | 2.3 | 1600 | 800 | 1600 | 800 | 800 | 400 | ≥3200 | < | < | 400 | ≥3200 | ≥3200 | < | < | < | < | < | |
| CP heron/HK/18/05 | 2.3 | < | < | < | < | < | < | < | 800 | < | ≥3200 | < | 1600 | < | < | < | < | 1600 | |
a
Abbreviations: FJ, Fujian; GD, Guangdong; Gs, goose; GX, Guangxi; GY, Guanyang; JX, Jiangxi; MDk, migratory duck; MYS, Malaysia; Qa, quail; Rb pochard, rosybilled pochard; SCk, silky chicken; SZ, Shenzhen (see also text).
b
Virus neutralization titers of ≥3200, 400 to 1600, and <400 (<) were viewed as indicating good, moderate, and poor reactivity, respectively. Clade classification of H5N1 viruses was based on phylogenetic genetic analysis of amino acids, as shown in Fig. 1. MAbs were grouped into three classes (I, II, and III) based on their reaction patterns in the cell-based neutralizing assay. ND, not done.