TABLE 1.
Experimental host range of MCLCuV determined by biolistic inoculation and infectivity based on symptom development and PCR amplification of the core CP fragmenta
| Test species | No. of infected plants/no. of inoculated plants | Symptoms phenotypeb | PCR result |
|---|---|---|---|
| Common bean (Phaseolus vulgaris L. “Top Crop”) | 7/17 | Chl, Lc | + |
| Cantaloupe (Cucumis melo L. “Hales Best Jumbo”) | 13/16 | Chl, Chls, Lc, St | + |
| Cotton (Gossypium hirsutum L. “DP 90”) | 0/18 | NS | − |
| Cucumber (Cucumis pepo L. “Bush Champion”) | 8/18 | Lc, Mo, St | + |
| Datura stramonium L. | 9/18 | Lc, Chls | + |
| Nicotiana benthamiana | 18/18 | Lc, Mo, St | + |
| Pepper (Capsicum annuum L. “Anaheim”) | 0/18 | NS | − |
| Pumpkin (Cucurbita maxima Duchnes “Big Max”) | 10/22 | Chl, Lc, St | + |
| Sweet basil (Ocimum basilcum) | 0/18 | NS | − |
| Tomato (Lycopersicum esculentum Mill. “Humaya”) | 0/28 | NS | − |
| Watermelon (Citrullus lanatus L. “Charleston Gray”) | 15/18 | Chl, Chls, Lc, Mo, St | + |
a
Tests included seven test plants/three replicates.
b
Chl, interveinal chlorosis; Chls, chlorotic spots; Lc, leaf curling; Mo, mosaic; St, stunting.