TABLE 2.

Viable reassortants in watermelon, bean, or pumpkin produced from heterologous combinations of the DNA-A or DNA-B component of MCLCuV-GT, BCaMV, CuLCrV-AZ, SLCV, and/or SMLCV and positive controls^a

Reassortant	DNA-B reassortant	No. of infected plants/no. of inoculated plants	Symptom phenotypeb	PCR result	Test host
DNA-A
BCaMV	MCLCuV	0/12	NS	−	Watermelon
CuLCrV	MCLCuV	0/12	NS	+	Watermelon
SLCV	MCLCuV	3/12	Chl, Chls	+	Watermelon
SMLCV	MCLCuV	0/12	NS	−	Watermelon
MCLCuV	BCaMV	0/12	NS	−	Watermelon
MCLCuV	CuLCrV	4/12	Chls	+	Watermelon
MCLCuV	SLCV	7/12	Chl, Lc, St	+	Watermelon
MCLCuV	SMLCV	1/12	NS	+	Watermelon
CuLCrV	MCLCuV	0/12	NS	−	Pumpkin
MCLCuV	CuLCrV	5/12	Chl, Lc	+	Pumpkin
MCLCuV	SMLCV	1/8	Chl, Lc	+	Pumpkin
SMLCV	MCLCuV	6/8	Chls, Lc	+	Pumpkin
MCLCuV	BCaMV	4/8	Chl, Lc	+	Bean
BCaMV	MCLCuV	0/8	NS	−	Bean
Homologous combinations and no-DNA controls
BCaMV DNA-A+B		0/6	NS	−	Watermelon
CuLCrV DNA-A+B		6/6	Chl, Chls, Lc, Mo, St	+	Watermelon
MCLCuV DNA-A+B		6/6	Chl, Chls, Lc, Mo, St	+	Watermelon
SLCV DNA-A+B		6/6	Chl, Lc, Mo, St	+	Watermelon
SMLCV DNA-A+B		0/6	NS	−	Watermelon
No-DNA control		0/24	NS	−	Watermelon
No-DNA control		0/8	NS	−	Pumpkin
No-DNA control		0/6	NS	−	Bean

^aPositive controls included wild-type virus or cognate components.

^bChl, interveinal chlorosis; Chls, chlorotic spots; Lc, leaf curling; Mo, mosaic; St, stunting; NS, no symptoms.