FIG. 4.
Cholesterol in the target cell is not required for EIAV infectivity. ED cells were treated with MβCD for 1 h and infected with EIAVMA-1 or EIAVvMA-1c or transduced with VSV-G- or EBOV-pseudotyped EIAV particles (A). EIAVMA-1 and EIAVvMA-1c viral particles or VSV-G-transducing particles were incubated with MβCD for 1 h. The particles were diluted into ED medium and used to infect ED cells (B). Cells were stained 40 h after infection and compared to the number of infected cells when particles were treated with the control. Data represent the averages and standard errors of three experiments performed in triplicate. *, P < 0.05; **, P < 0.001.