FIG. 3.

Gag and Pol proteins expressed in the Gag PTC mutants. Mutant proviruses were transfected into 293T cells, proteins were extracted 2 days posttransfection, and the Western blot was probed with anti-Gag (A) or anti-Pol (B) antibody. Lane 2 was transfected with the pGag plasmid alone. In lanes 8 to 12, the wt or C-terminally truncated Gag mutants were cotransfected with pGag at a 1:1 molar ratio. The open circles indicate the C-terminally truncated Gag proteins expressed by mutants.