FIG. 7.

(A) Sequence of the ASLV ESE introduced downstream of the PTC mutation in the GR (−)st mutant, as well as in the wt. (B) Levels of mRNA measured by RT-PCR. 293T cells were transfected with the proviral vectors encoding ESE either in a wt (wt/ESE) or in a GR (−)st mutant [(−)st/ESE] background. Total cellular RNA was extracted to prepare cDNA and run on PCR amplification using the primers specific for unspliced or spliced mRNAs shown in Fig. 4A. The levels of gapdh mRNA were used to normalize the RNA before reactions were run.