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Quantitation of GR st mutant mRNA levels by real-time PCR
| Construct | Levela
|
Ratio of spliced to unspliced | |
|---|---|---|---|
| Spliced | Unspliced | ||
| Mock | ND | ND | |
| Wt | 1.00 | 1.00 | 1.0 |
| GR (−)st | 0.05 ± 0.01 | 1.62 ± 0.40 | 0.03 |
| GR 1st | 0.42 ± 0.20 | 3.38 ± 0.56 | 0.12 |
| GR 2st | 0.04 ± 0.01 | 1.83 ± 0.11 | 0.02 |
The levels of mutant mRNA are normalized to that of the wt. The numbers are averages ± standard deviations from three independent assays. ND, not detected.
