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Quantitation of GR (−)st missense mutant mRNA levels by real-time PCR
| Construct | Levela
|
Ratio of spliced to unspliced | |
|---|---|---|---|
| Spliced | Unspliced | ||
| Mock | ND | ND | |
| Wt | 1.00 | 1.00 | 1.0 |
| GR (−)st | 0.03 ± 0.01 | 0.95 ± 0.20 | 0.03 |
| m-GTG | 0.81 ± 0.18 | 1.03 ± 0.21 | 0.79 |
| m-ATC | 1.44 ± 0.38 | 1.01 ± 0.18 | 1.43 |
| m-GCA | 0.50 ± 0.11 | 0.77 ± 0.31 | 0.65 |
The levels of mutant mRNA are normalized to that of the wt. The numbers are averages ± standard deviations from four independent assays. ND, not detected.
