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Quantitation of mRNA levels in the presence of ESE by real-time PCR
| Construct | Levela
|
Ratio of spliced to unspliced | |
|---|---|---|---|
| Spliced | Unspliced | ||
| Mock | ND | ND | |
| Wt | 1.00 | 1.00 | 1.0 |
| GR (−)st | 0.03 ± 0.01 | 0.95 ± 0.20 | 0.03 |
| Wt/ESE | 8.43 ± 1.50 | 0.60 ± 0.22 | 14.1 |
| (−)st/ESE | 1.52 ± 0.30 | 0.31 ± 0.14 | 4.9 |
The levels of mutant mRNA are normalized to that of the wt. The numbers are averages ± standard deviations from three independent assays. ND, not detected.
