FIG. 2.

Nup98 is cleaved very rapidly after infection. (A) Portions (50 μg) of whole-cell lysates prepared from mock-infected cells or cells that had been infected with poliovirus for the indicated lengths of time were analyzed by immunoblotting with polyclonal antisera to Nup98 or eIF4GI or monoclonal antibodies to detect Nup153 or nucleolin. The positions of the N-terminal eIF4GI cleavage products and molecular mass markers are indicated in kilodaltons. All antibodies were used at 1:5,000 dilutions, and the exposure times were 5 min for Nup98 and 10 s for Nup153, eIF4GI, and nucleolin. For the percent remaining values, band intensities were quantitated by densitometry, and the amounts relative to mock-infected cells are indicated. (B) A 30-min exposure of the immunoblot shown in panel A, with the putative Nup98 cleavage products indicated.