FIG. 3.

Effect of guanidine on cleavage of NPC proteins. Portions (50 μg) of whole-cell lysates prepared from mock-infected cells or cells that had been infected with poliovirus for 2 or 5 h were analyzed by immunoblotting with the indicated antibodies. “Guanidine” refers to cells treated with 2 mM GuHCl. “Cycloheximide” refers to cells treated with 100 μg of cycloheximide/ml. Mock-infected cells treated with cycloheximide or guanidine were exposed to the reagent for 5 h. All antibodies were used at 1:5,000 dilutions, and exposure times were 1 min for Nup98, Nup153, and Nup62 and 10 s for eIF4GI and nucleolin. For the percent remaining values, band intensities were quantitated by densitometry, and the amounts relative to mock-infected cells are indicated.