FIG. 6.

Colocalization and coaggregation of ICP4 and ICP0 in the cytoplasm of ΔgE or ΔgI mutant virus-infected cells. HEL cells seeded in four-well slides were either mock infected (a, b, and c) or exposed (10 PFU/cell) to HSV-1(F) (d, e, and f), ΔgE (g, h, and i), or ΔgI (j, k, and l) mutant virus. At 9 h after infection, the cells were fixed in 4% paraformaldehyde and doubly stained with the mouse monoclonal antibody to ICP4 and the rabbit polyclonal antibody to ICP0 exon II, followed by reactions with the goat anti-mouse antibody conjugated to Alexa Fluor 488 (green fluorescence) and the goat anti-rabbit antibody conjugated to Alexa Fluor 594 (red fluorescence), respectively, as detailed in Materials and Methods. The images were captured as described in the legend to Fig. 4.