FIG. 7.

The rate of reoccupation of HSP promoters with nucleosomes during recovery from heat shock is the same for all three promoters and is delayed in the ΔSNF2 strain. (A) ChIPs using antibody against the C terminus of histone H3 were performed with the ΔSNF2 or isogenic WT strain. The promoter analyzed is indicated in each panel. y axis, n-fold displacement of histone H3. For the x axis, the first value is for the initial 30°C culture, the second value is for the culture incubated for 16 min at 39°C, and the following time points (0.5 to 64 min) are after a downshift of the temperature from 39°C to 30°C. All real-time PCR values were normalized relative to the input and to the signal from the PHO5 promoter, similar to results in Fig. 2. (B) Superimposure of data from panel A for WT and ΔSNF2 strains is shown.