FIG. 2.

Overexpression of Hu proteins in HeLa and PC12 cells reduces inclusion of the endogenous NF1 exon 23a. (a) Transient transfection of PTB or mHuC in HeLa cells. Inclusion of endogenous NF1 exon 23a was detected by RT-PCR using total RNA isolated from the transfected cells and the oligonucleotides shown in Fig. 1a. The level of overexpressed proteins was detected by Western blot analysis. (b) Stable transfection of PTB or mHuC in PC12 cells. Inclusion of endogenous NF1 exon 23a was detected by RT-PCR. Error bars indicate standard deviations.