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. 2007 Dec 17;28(4):1240–1251. doi: 10.1128/MCB.01509-07

FIG. 8.

FIG. 8.

U2AF65 cross-linking with the RNA substrate indicated in the diagram. The 32P-labeled, in vitro-transcribed RNA transcripts were UV cross-linked in HeLa cell nuclear extract in the presence of increasing amounts of buffer alone or of GST-mHuB RRM1,2 or mHuB (50 and 200 ng) and immunoprecipitated with the anti-U2AF65 antibody. nt, nucleotides.