TABLE 1.

Oligonucleotides used in this study

Oligonucleotide	Sequence	Restriction site
NF1 5′-1	GACAGATCTAGATGAAACCAATATGTGCC	BglII
NF1 5′-2	GACAGATCTGTACAAGCCAACATT	BglII
NF1 3′-1	GCGGATCCAGACTGCCTAGATAAAAATA	BamHI
NF1 5′-3	AGAAGCTTCATTGTTTTTGTTGCTGTAT	HindIII
NF1 3′-2	ATGAATTCATTTTACAGTGAAGGTCA	EcoRI
mHuB 5′	GCGGATCCAATGGAAACACAACTGTCTAA	BamHI
mHuB 3′	GCGAATTCTTAGGCTTTGTGCGTTTTGTT	EcoRI
mHuC 5′	GCGGATCCAATGGTCACTCAGATACTGGG	BamHI
mHuC 3′	GCGAATTCTCAGGCCTTGTGCTGCTTGC	EcoRI
DS8	TTGACCATTCACCACATTGGTGTGC
HMT3	ATCTGGGAGCGGGGCTGT
NF1 5′-4	AAGTTCTTCCATGCCATCATCAG
NF1 3′-3	ATTCTAGGTGGTGGCTTTTTATCTA
NF1 5′-5	AAGGGACATCTTCAAAGA
NF1 3′-4	TTTATGTACAAGCCAACA
GAPDH 5′	ACCTCCAAACTGAAGAGC
GAPDH 3′	TGGCAGGTTTCTCCAGGCGGC