TABLE 2.
Observed synthetic interactions between SPN1 and specific gene products involved in RNAPII-mediated transcription
| Protein category and deleted gene | Change in phenotype under the following conditiona:
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| YPD
|
1 M NaCl | 1 M sorbitol | 4 mM H2O2 | Ino− | YPGal | YPEG | 50 mM AT | MPA | |||
| 30°C | 38°C | 14°C | |||||||||
| Subunits of Swi/Snf complex | |||||||||||
| SNF2b | Sup | Sup | N | Sup | Sup | Sup | Sup | Sup | Sup | Sup | Sup |
| SNF5 | N | Sup | N | N | N | Sup | Sup | Sup | Sup | Sup | Sup |
| SNF6 | N | Sup | N | N | N | Sup | Sup | Sup | Sup | Sup | Sup |
| Elongation factors | |||||||||||
| DST1 | N | N | N | Ex | Ex | Ex | N | Ex | Ex | Ex | Ex |
| RTF1 | N | N | N | Ex | Ex | Ex | N | Ex | Ex | Ex | Ex |
| SPT4 | SL | SL | SL | SL | SL | SL | SL | SL | SL | SL | SL |
a
Deletion strains (as indicated) were combined with the K192N derivative of SPN1, and alterations in growth properties under 11 different conditions were assayed. N, no change in phenotype from that observed in either of the two parental strains (the knockout strain or the K192N strain); Ex, phenotype exacerbated in the double mutant strains; Sup, suppression of mutant phenotypes in the double mutant background; SL, synthetic lethality in the double mutant background.
b
Synthetic interactions between SNF2 and SPN1 were observed by mutating SPN1 in strain CMKy42 (15).