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. 2007 Dec 17;28(4):1413–1426. doi: 10.1128/MCB.01301-07

FIG. 5.

FIG. 5.

The BAF250a interaction with BRG1 requires the HSA domain. (A) Schematic of wild-type BRG1 indicating the region required for interaction with BAF250a. (B) Wild-type BRG1 was immunopurified from BRG1-transfected SW-13 cells using antibodies specific for proteins reported to interact over the BRG1 HSA region (BAF250a) or core BAF subunits (BAF155 and BAF170). Immunoprecipitates (IP) were resolved by 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by immunoblotting (IB) using the indicated antibodies (Ab). Normal IgG was used to detect nonspecific interactions. (C) Complexes containing BRG1 and mutant BRG1 were immunopurified from transfected SW-13 cells using antibodies specific for BAF250a, V5 epitope, and BRG1 (IP Ab). Immunoprecipitates were resolved by 6% SDS-PAGE and analyzed by immunoblotting (IB) using anti-V5 or anti-BAF250a antibodies. Normal IgG was used to detect nonspecific interactions.