FIG. 7.

BAF250a protein is required for expression of endogenous BRG1-dependent and GR-responsive BRG1-dependent genes. (A) Real-time reverse transcription-PCR analysis was used to determine the expression level of endogenous BRG1-dependent genes CD44 or CRYAB in wild-type SW-13 cells cotransfected with BRG1 expression plasmid and BAF250a siRNA duplexes. Equal amounts of cDNA were used for real-time PCR analysis with primers specific for CD44 or CRYAB. (B) Total RNA, extracted from Dex- or vehicle-treated SW-13/MMTV cells cotransfected with GR and BRG1 expression vectors and BAF250a siRNA duplexes, was used as template for cDNA synthesis. Equal amounts of cDNA were used for real-time PCR analysis with primers specific for PLZF or HSD-11β type 2. Quantitative analysis was performed using Mx3000P software (Stratagene). Data were normalized to GAPDH and displayed as relative transcription. Values are shown as means plus standard deviations (error bars) from three experiments. EtOH, ethanol.