PFGE analysis for trt1-D743A and taz1Δ trt1-D743A cells. A taz1Δ/taz1+ trt1-D743A/trt1+ diploid strain was sporulated, and trt1-D743A (A to C) or taz1Δ trt1-D743A (D to F) cells were selected. (A and D) Liquid growth characteristics of cells after germination. For references, results from growth curve experiments for wild-type and taz1Δ cells are also plotted (dotted lines). (B and E) PFGE of NotI-digested S. pombe chromosomal DNA hybridized with telomere probe. (C and F) PFGE of NotI-digested S. pombe chromosomal DNA hybridized with probes for the C, I, L, and M bands.