FIG. 1.

PR-mediated induction of PPARγ in the ovary during ovulation. Age-matched prepubertal WT (+/+) and PR-null (−/−) mice were treated with PMSG for 48 h prior to stimulation of ovulation by hCG. The ovaries were collected from these mice at 0 h (48 h PMSG) and 5 h after administration of hCG. Total RNA was isolated and converted by reverse transcription to cDNA. The cDNA was analyzed by real-time quantitative PCR using primer sets specific for PPARγ (A) and COX-2 (B), respectively. The expression of 36B4 mRNA, encoding a ribosomal protein, was used to normalize the variability of mRNA amounts in the samples.