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. 2008 Jan 2;28(5):1770–1782. doi: 10.1128/MCB.01556-07

FIG. 2.

FIG. 2.

Localization of PPARγ in the ovary and its regulation by PR. (A) Spatial expression of PPARγ mRNA during ovulation. In situ hybridization was performed by using sense and antisense RNA probes corresponding to PPARγ cDNA to examine its expression in the ovaries of prepubertal WT (+/+) and PR-null (−/−) mice at 0 and 5 h after administration of hCG following a 48-h treatment with PMSG. Signals corresponding to the antisense RNA probe are shown. No signal for PPARγ mRNA was seen when the sense RNA probe was used. (B) Examination of PR regulation of PPARγ mRNA in the ovary during ovulation. Ovaries were collected from prepubertal WT (+/+) and PR-null (−/−) mice at 0 and 5 h after administration of hCG following a 48-h treatment with PMSG. The tissues were processed and subjected to in situ hybridization as described in Materials and Methods. (C) Analysis of PPARγ protein expression in the ovary. Ovaries from WT (0 and 5 h after administration of hCG) and PR-null (5 h after administration of hCG) mice were processed and subjected to immunohistochemical analysis using a rabbit polyclonal anti-PPARγ antibody as described in Materials and Methods.