FIG. 7.

A PPARγ-selective antagonist inhibits the expression of several PR target genes in primary granulosa cells. Primary granulosa cells were extracted from the ovaries of prepubertal mice primed with PMSG for 48 h and were cultured overnight in 1% fetal bovine serum-DMEM-F12. The cells were then treated with forskolin (Fo; 10 μM) and PMA (20 nM) to activate LH signaling in the presence or absence of GW9662 (1 μM), a PPARγ-selective antagonist. Total RNA was isolated from these cells, and the expression of mRNAs corresponding to ET-2 (A), cGKII (B), and IL-6 (C) was then measured by real-time quantitative reverse transcription-PCR using gene-specific primers as described in Materials and Methods.