FIG. 9.

Repression of PPARγ target genes by a COX-2-selective inhibitor is reversed by rosiglitazone. Primary granulosa cells were isolated from the ovaries of prepubertal mice primed with PMSG for 48 h. The cells were stimulated with forskolin (10 μM) and PMA (20 nM) in the presence or absence of NS-398 (10 μM), a COX-2-specific inhibitor. Rosiglitazone (1 or 5 μM), a PPARγ-specific agonist ligand, was added as indicated. Gene expression was analyzed by real-time quantitative PCR using gene-specific primers for ET-2 (A), cGKII (B), and IL-6 (C), respectively.