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Nucleotide binding is required for Monarch-1 self association. HEK293T cells were transfected with full-length forms of HA- or Flag-tagged WT or mutA/B Monarch-1. Flag-tagged proteins were immunoprecipitated (IP) with an anti-Flag antibody, and Western blots (IB) were probed with anti-HA antibody to detect self-associated Monarch-1.
