FIG. 1.

Interaction between endogenous βPix and 14-3-3 isoforms. HEK293 cells were treated with IBMX (200 μM) for 30 min and then stimulated with forskolin (20 μM) for 15 min in the presence or absence of myristoylated PKI (2 μM). Cells lysates were subjected to immunoprecipitation using anti-βPix antibody, and the membranes were blotted using antibodies against 14-3-3β and -γ (A) or 14-3-3ζ and -ɛ (B). Immunoprecipitated 14-3-3 and bound βPix were detected using anti-14-3-3s and anti-βPix antibodies, respectively. TCL, total cell lysate.