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. 2007 Dec 26;28(5):1679–1687. doi: 10.1128/MCB.00898-07

FIG. 2.

FIG. 2.

Identification of 14-3-3β binding site on β1Pix. (A) Lysates from cells expressing β1Pix, β1Pix(S560A), β1Pix(S516A, T526A), or β1Pix(S516E, T526E) were immunoprecipitated with anti-14-3-3β and subjected to Western blot analysis with anti-Myc or anti-14-3-33-3β. (B) Lysates from cells expressing β1Pix, β1PixΔ(547-586), β1PixΔ(587-626), or β1PixΔ(602-611) were immunoprecipitated with anti-14-3-3β, and Western blots were analyzed as indicated above. (C) Cells expressing β1Pix, β1Pix(S516A, T526A), or β1PixΔ(602-611) were immunoprecipitated with anti-14-3-3β, and the Western blots were revealed as indicated above. The experiment was repeated two additional times with similar results.