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. 2007 Dec 17;28(5):1802–1814. doi: 10.1128/MCB.00201-07

FIG. 5.

FIG. 5.

Pyk2 is potential effector to Wrch1. (A) Normal MEFs were transfected with Wrch1, Wrch1(T63N), Pyk2, Pyk2 plus Wrch1 (Wrch1wt+Pyk2), or Pyk2 plus Wrch1(T63N) (Wrch1/T63N+Pyk2). Myc-tagged Wrch1 was detected by a rabbit anti-Myc antibody followed by an AMCA-conjugated anti-rabbit antibody. Pyk2 was detected by a mouse anti-Pyk2 antibody followed by a TRITC-conjugated anti-mouse antibody. Filamentous actin was detected by Alexa Fluor 488-conjugated phalloidin. The arrowheads mark transfected cells. Bar, 20 μm. (B) Quantification of the phenotypes observed in panel A was performed by microscopy analysis and scored as filopodium formation (filopodia) or stress fiber dissolution (SF loss). The values represent analyses of at least 100 transfectants in three independent experiments. Wrch1wt, wild-type Wrch1.