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. 2007 Dec 26;28(5):1713–1723. doi: 10.1128/MCB.01360-07

FIG. 1.

FIG. 1.

Expression of stabilized β-catenin in thymocytes causes growth arrest and cellular senescence. (A) Abundance of human (transgenic) β-catenin mRNA relative to GAPDH in sorted CAT-Tg DN2, DN3, and DN4 cells analyzed by real-time PCR (n = 3). Shown is Western blot analysis of endogenous murine (upper bands) and transgenic human (lower bands) β-catenin protein (representative of data from five mice). (B) BrdU incorporation in electronically gated DN4 thymocytes from C57BL/6 and CAT-Tg mice. The graph shows average data from more than three mice for each genotype in one experiment and is representative of three experiments. (C) Real-time RT-PCR analysis of the expression of cell cycle regulators in sorted DN4 thymocytes from C57BL/6 (n = 3) and CAT-Tg (n = 3) mice. (D) Real-time PCR analysis of SA genes in sorted DN4 thymocytes from C57BL/6 and CAT-Tg mice (n = 3). (D) SA β-galactosidase activity in thymic cryosections from C57BL/6 and CAT-Tg mice (representative of n = 4).