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. 2007 Dec 26;28(5):1713–1723. doi: 10.1128/MCB.01360-07

FIG. 2.

FIG. 2.

Enhanced DNA damage and p53-dependent apoptosis in CAT-Tg mice. (A) Immunofluorescence staining of phosphorylated histone γH2AX in fixed DN (eight CAT-Tg and C57BL/6 mice) and DP (two CAT-Tg and two C57BL/6 mice) thymocytes. The bar graph shows the percentage of γH2AX-positive cells in C57BL/6 DN and CAT-Tg DN thymocytes (see Fig. S3 in the supplemental material). (B) Three examples of γH2AX foci in CAT-Tg DN thymocytes shown at a higher magnification. (C) Annexin V staining of DN4 thymocytes (electronically gated) from C57BL/6 and CAT-Tg mice. The numbers in the graph are an average of data from more than three mice for each genotype in one experiment that is representative of six experiments. (D) Real-time RT-PCR analysis of the expression of p53 pathway-associated genes in sorted DN4 thymocytes from CAT-Tg (n = 3) and C57BL/6 (n = 3) mice.