FIG. 2.

Inactivation of HDAC6 in vivo leads to tubulin hyperacetylation. (A) Absence of HDAC6 protein in HDAC6-deficient mice. Protein extracts from either HDAC6 wild-type (WT) or deficient (knockout [KO]) male mice were analyzed by Western blotting with an antibody against HDAC6. The mouse HDAC6-specific band is marked by an arrow. The strong band in the liver sample is a cross-reacting protein. (B) Tubulin hyperacetylation in HDAC6-deficient mice. Protein extracts from the indicated organs were immunoblotted for α-tubulin as a loading control and acetylated tubulin to analyze tubulin acetylation.